Large?Scale Fabrication of 3D Scaffold?Based Patterns of Microparticles and Breast Cancer Cells using Reusable Acoustofluidic Device

Spatial distribution of biological cells plays a key role in tissue engineering for reconstituting the cellular microenvironment, and recently, acoustofluidics are explored as viable tool controlling structures fabrication because its good biocompatibility, low-power consumption, automation capability, nature non-invasive, non-contact. Herein, reusable acoustofluidic device is developed using surface acoustic waves manipulating microparticles/cells to form 3D matrix pattern inside scaffold-based hydrogel contained millimetric chamber. The patterned polymerized construct can be easily safely removed from chamber proposed lifting technique, which prevent any physical damages or contaminations promote reusability generated patterns microparticles numerically studied finite-element method, well validated by experimental results. useful vitro artificial tissues drug toxicity testing building organs-on-chip applications.